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KMID : 0380020130280050332
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 5 p.332 ~ p.337
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Comparison of Growth Inhibitory Effects on Cancer Cells of Saponin and Fucoidan Treated with Recombinant Thermophilic Xylose Isomerase
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Lee Dong-Geun
Park Seong-Hwan
Lee Sang-Hyeon
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Abstract
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The gene encoding for xylose isomerase from the thermophilic bacterium Thermotoga maritima was cloned and recombinantly expressed in E. coli cells. Optimal activity was shown at 90oC and pH 8.0. Treatment of saponin by recombinant xylose isomerase increased the growth inhibitory effect against human gastric cancer (AGS) cells and human colon cancer (HT-29) cells. On the other hand, treatment of fucoidan by the enzyme could not change the growth inhibitory effect against the same cancer cells. One μg/ml of enzyme-treated saponin exhibited the same or higher growth inhibitory effect against both cancer cells compared with 100 μg/ml of enzymeuntreated saponin. These results would be useful in the development of functional food or drug.
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KEYWORD
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Cancer cells, Fucoidan, Saponin, Thermotoga maritima, Xylose isomerase
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